KLAST is a fast, accurate and NGS scalable bank-to-bank sequence similarity search tool providing significant accelerations of seeds-based heuristic comparison methods, such as the Blast suite of algorithms.

Relying on unique software architecture, KLAST takes full advantage of recent multi-core personal computers without requiring any additional hardware devices.


KLAST's major features for next-generation sequence data analysis

KLAST is a general purpose sequence comparison tool providing the following benefits.

  • KLAST is the professional version of the high-performance Parallel Local Sequence Alignment Search Tool published in BMC Bioinformatics,
  • Reduces the processing time of sequences comparisons (up to 25 times faster than Blast) while providing highest quality results,
  • Designed to compare two large sets of sequence databanks in a single run,
  • Contains a fully integrated data filtering engine capable of selecting relevant hits with user-defined criteria (E-Value, identity, coverage, alignment length, etc.),
  • Does not require any additional hardware, since it is a software solution. It is easy to install, cost-effective, takes full advantage of multi-core processors and uses a small RAM footprint,
  • Provides an integrated algorithm suite which automatically processes analysis workflows that includes similarity searches, hits annotations, and data filtering.

KLAST use cases in NGS applications

Fast and accurate sequence similarity search tool

KLAST is fully designed to compare query and subject comprised of large sets of DNA, RNA and protein sequences using KLASTn, KLASTp, KLASTx, tKLASTx and tKLASTn methods.

KLAST is significantly faster than original PLAST, while providing comparable sensitivity to BLAST and SSearch algorithms.

KLAST contains a fully integrated data filtering engine capable of selecting relevant hits with user-defined criteria (E-Value, identity, coverage, alignment length, etc.).

Read more about the KLAST algorithm

KLAST benchmark

 Read more about KLAST benchmarks and use cases 
  Klast, Blast, SSearch comparison This benchmark illustrates the optimal speed/quality ratio provided by KLAST over standard tools  
  Plant Genome
Annotation
Discover how to run a full plant genome annotation in only half a day using KLAST vs. more than 7 days using Blast+  
  Tara Oceans
Metagenomics
A use case of KLAST on metagenomics data analysis applied to the Tara Oceans International Project  
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