ngKLAST 4.7 is an all-in-one KLAST and BLAST workstation to produce quickly and efficiently high-quality results out of any kind of sequences: proteins, genes, reads, contigs, genomes, metagenomes, transcriptomes, etc.

ngKLAST can be used for metagenomics, microbial communities analysis, full genome / proteome annotation and comparison, transcriptome annotation, functional and structural studies of proteins, taxonomy & phylogenetic analyses and microarray annotations, among others.

ngKLAST is a software providing convenient tools to analyze, align and compare genetics and genomics sequences, as well as metagenomics and next-generation sequencing data (NGS). ngKLAST includes the KLAST high-performance sequence similarity search tool, as well as easy ways to use BLAST services: local (i.e. on your computer), NCBI & EBI public servers, NCBI BLAST machine on Amazon Cloud and Korilog Cloud:

With an easy-to-use workflow engine and a unified set of data viewers, the ngKLAST platform makes sequence databanks exploration accessible to every life science researcher. Sequence similarity search results can be enriched with biological classification data, such as NCBI Taxonomy, Gene Ontology and Enzyme.

Main features

Databank Installation

  • Automatic deployment from remote servers
  • Install public databanks (NCBI, EMBL, Uniprot,...)
  • Prepare personal databanks
  • Manage sequences, annotations and biological classifications
  • Prepare taxonomic subsets from public databanks

Databank Search (KLAST and BLAST search)

Comparison of local KLAST, local BLAST, Amazon BLAST and NCBI Web BLAST

Data Visualization

  • Sequences, 3D structures
  • Sequence Alignments (pairwise and multiple)
  • Tables, phylogenetic trees, position specific matrices
  • Conserved regions
  • Features, annotated Hit 2D plots
  • Biological Classification (NCBI Taxonomy, Gene Ontology, Enzyme, ...); NCBI Taxonomy: Lowest Common Ancestor (LCA) analysis
Analysis of a bacterial metagenome data set.

Data Management

  • Analyse data using convenient viewers
  • Export data (text, spreadsheet, images, ...)
  • Organize data safely over time
  • Share data with other users

Databank Query

  • Sequences (nucleotides, proteins)
  • 3D structures
  • Query local and remote (NCBI, RSCB) services
  • Batch retrieval of sequences
  • Direct BLAST and KLAST submission


Practical use cases of ngKLAST on next-generation sequencing data (NGS)

ngKLAST can be used for:

Have a look at the ngKLAST videos, or contact us to discuss with you the opportunities of using ngKLAST for your research work.


Academic Free vs. Professional releases

A Free version of ngKLAST is available at no charge for users from academic and non-profit institutions. The Free version enables the use of BLAST from remote public servers only.

The Professional version does not have any limitations and comes with advanced features, including local search services, no limit on query size, use of personal and custom-made databanks and additional data analysis viewers.

The following table gives a quick overview of the main differences between Free and Professional releases of ngKLAST:

  Cores max 4 / computer up to 16 / computer  
  Query size max 2,500 queries / batch no limit  
  Reference databanks public only Public, personal and user-made  
  Remote BLAST (NCBI, EBI, Amazon Cloud)  
  Local BLAST -  
  Local KLAST -  
  BLAST and KLAST on KServer -  
  Use your databanks:
+ Databank Manager
+ Custom-made databanks
  Data Integration (GO, Enzyme, Taxonomy; LCA computation) From NCBI Server (1) High-performance (2)  
  Map Viewer, Genome Browser, 3D Viewer, MSA Editor -  

(1) According to NCBI requirements for the use of their public resources, ngKLAST will delay the data retrieval process over time. Retrieval time can range from a few seconds to several hours. (2) However, using ngKLAST Professional with either local databanks or KServer, data retrieval has no impact on search time.


Boost your work in your lab with the Korilog Server

ngKLAST can be used with the Korilog Server (or KServer). This software enables the use of a dedicated computer infrastructure to run BLAST and KLAST jobs (Blast or Klast comparison + data retrieval + data filtering). The server software is compatible with various jobs scheduling system such as SGE/OGE, PBS-Pro, LSF and SLURM.

Here is a snapshot of the two main solutions available to deploy the Korilog server:

KLAST Server - Architecture  - sequence search alignment structure tree phylogenetic bioinformatics genomics proteomics Software design bioinformatic analysis biological databanks biological information systems



Boost even more your work with Korilog HPC Cloud


To enable you to save time and money and to avoid you to worry about installing and maintaining computer nodes, softwares and sequence databanks in your lab, we provide a dedicated and secured high-performance cloud computing infrastructure to run KLAST and BLAST jobs of any size, including massive NGS comparisons.

Using the ngKLAST client, you can easily and efficiently connect to Korilog cloud solution using a cost-effective pay-per-use mode.

Read more about Korilog Cloud Solution.




Try ngKLAST for free

Download ngKlast


Korilog softwares are available for use on Linux, Mac and Windows platforms.

All recent Linux systems should be fine Intel or AMD 64 bits processors
Windows Seven or above. Intel or AMD 64 bits processors
MacOS-X 10.7 or above. Intel 64 bits processors.
ngKLAST release notes

ngKLAST release notes are available here.

Download ngKLAST product sheet

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