ngKLAST 4.5 is an all-in-one KLAST and BLAST workstation to produce quickly and efficiently high-quality results out of any kind of sequences: proteins, genes, reads, contigs, genomes, metagenomes, transcriptomes, etc.
ngKLAST is a software providing convenient tools to analyze, align and compare genetics and genomics sequences, as well as metagenomics and next-generation sequencing data (NGS). ngKLAST includes the KLAST high-performance sequence similarity search tool, as well as easy ways to use BLAST services: local (i.e. on your computer), NCBI & EBI public servers and NCBI BLAST machine on Amazon Cloud:
With an easy-to-use workflow engine and a unified set of data viewers, the ngKLAST platform makes sequence databanks exploration accessible to every life science researcher. Sequence similarity search results can be enriched with biological classification data, such as NCBI Taxonomy, Gene Ontology and Enzyme.
|Comparison of local KLAST, local BLAST, Amazon BLAST and NCBI Web BLAST|
ngKLAST can be used for:
A Free version of ngKLAST is available at no charge for users from academic and non-profit institutions. The Free version enables the use of BLAST from remote public servers only.
The Professional version does not have any limitations and comes with advanced features, including local search services, no limit on query size, use of personal and custom-made databanks and additional data analysis viewers.
The following table gives a quick overview of the main differences between Free and Professional releases of ngKLAST:
|Cores||max 4 / computer||up to 16 / computer|
|Query size||max 2,500 queries / batch||no limit|
|Reference databanks||public only||Public, personal and user-made|
|Remote BLAST (NCBI, EBI, Amazon Cloud)|
|BLAST and KLAST on KServer||-|
|Use your databanks:
+ Databank Manager
+ Custom-made databanks
|Data Integration (GO, Enzyme, Taxonomy; LCA computation)||From NCBI Server (1)||High-performance (2)|
|Map Viewer, Genome Browser, 3D Viewer, MSA Editor||-|
(1) According to NCBI requirements for the use of their public resources, ngKLAST will delay the data retrieval process over time. Retrieval time can range from a few seconds to several hours. (2) However, using ngKLAST Professional with either local databanks or KServer, data retrieval has no impact on search time.
ngKLAST can be used with the KLAST Server (or KServer). This software enables the use of a dedicated computer infrastructure to run BLAST and KLAST jobs (Blast or Klast comparison + data retrieval + data filtering). The server software is compatible with SGE/OGE, PBS-Pro, LSF and SLURM jobs scheduling systems.
Here is a snapshot of the two main solutions available to deploy the KLAST server:
Korilog softwares are available for use on Linux, Mac and Windows platforms.
|All recent Linux systems should be fine Intel or AMD 64 bits processors|
|Windows Seven or above Intel or AMD 64 bits processors|
|MacOS-X 10.6 or above. Intel 64 bits processors.|