ngKLAST 4.7 is an all-in-one KLAST and BLAST workstation to produce quickly and efficiently high-quality results out of any kind of sequences: proteins, genes, reads, contigs, genomes, metagenomes, transcriptomes, etc.
ngKLAST can be used for metagenomics, microbial communities analysis, full genome / proteome annotation and comparison, transcriptome annotation, functional and structural studies of proteins, taxonomy & phylogenetic analyses and microarray annotations, among others.
ngKLAST is a software providing convenient tools to analyze, align and compare genetics and genomics sequences, as well as metagenomics and next-generation sequencing data (NGS). ngKLAST includes the KLAST high-performance sequence similarity search tool, as well as easy ways to use BLAST services: local (i.e. on your computer), NCBI & EBI public servers, NCBI BLAST machine on Amazon Cloud and Korilog Cloud:
With an easy-to-use workflow engine and a unified set of data viewers, the ngKLAST platform makes sequence databanks exploration accessible to every life science researcher. Sequence similarity search results can be enriched with biological classification data, such as NCBI Taxonomy, Gene Ontology and Enzyme.
|Comparison of local KLAST, local BLAST, Amazon BLAST and NCBI Web BLAST|
|Analysis of a bacterial metagenome data set.|
ngKLAST can be used for:
A Free version of ngKLAST is available at no charge for users from academic and non-profit institutions. The Free version enables the use of BLAST from remote public servers only.
The Professional version does not have any limitations and comes with advanced features, including local search services, no limit on query size, use of personal and custom-made databanks and additional data analysis viewers.
The following table gives a quick overview of the main differences between Free and Professional releases of ngKLAST:
|Cores||max 4 / computer||up to 16 / computer|
|Query size||max 2,500 queries / batch||no limit|
|Reference databanks||public only||Public, personal and user-made|
|Remote BLAST (NCBI, EBI, Amazon Cloud)|
|BLAST and KLAST on KServer||-|
|Use your databanks:
+ Databank Manager
+ Custom-made databanks
|Data Integration (GO, Enzyme, Taxonomy; LCA computation)||From NCBI Server (1)||High-performance (2)|
|Map Viewer, Genome Browser, 3D Viewer, MSA Editor||-|
(1) According to NCBI requirements for the use of their public resources, ngKLAST will delay the data retrieval process over time. Retrieval time can range from a few seconds to several hours. (2) However, using ngKLAST Professional with either local databanks or KServer, data retrieval has no impact on search time.
ngKLAST can be used with the Korilog Server (or KServer). This software enables the use of a dedicated computer infrastructure to run BLAST and KLAST jobs (Blast or Klast comparison + data retrieval + data filtering). The server software is compatible with various jobs scheduling system such as SGE/OGE, PBS-Pro, LSF and SLURM.
Here is a snapshot of the two main solutions available to deploy the Korilog server:
To enable you to save time and money and to avoid you to worry about installing and maintaining computer nodes, softwares and sequence databanks in your lab, we provide a dedicated and secured high-performance cloud computing infrastructure to run KLAST and BLAST jobs of any size, including massive NGS comparisons.
Using the ngKLAST client, you can easily and efficiently connect to Korilog cloud solution using a cost-effective pay-per-use mode.
Korilog softwares are available for use on Linux, Mac and Windows platforms.
|All recent Linux systems should be fine Intel or AMD 64 bits processors|
|Windows Seven or above. Intel or AMD 64 bits processors|
|MacOS-X 10.7 or above. Intel 64 bits processors.|