Expression, Purification and Characterization of the Hepatitis E Virus Like-Particles in the Pichia pastoris.

Expression, Purification and Characterization of the Hepatitis E Virus Like-Particles in the Pichia pastoris.
Hepatitis E virus (HEV) is related to acute hepatitis illness, which can result in power illness in immunocompromised people. The illness is especially extreme amongst pregnant girls (20-30% mortality).
The one licensed vaccine towards HEV, which is offered in China, is the Escherichia coli purified recombinant virus-like particles (VLPs) encompassing the 368-660 amino acids (aa) of the viral ORF2 protein. The viral capsid is fashioned by the ORF2 protein, which harbors three glycosylation websites.
 Baculo virus expression system has been employed to generate a glycosylated VLP, which encompasses 112-608aa of the ORF2 protein. Right here, we sought to supply a recombinant VLP containing 112-608aa of the ORF2 protein in Pichia pastoris (P. pastoris) expression system.
The cDNA sequence encoding 112-608aa of the ORF2 protein was fused with the α-mating issue secretion sign coding sequence (for launch of the fusion protein to the tradition medium) and cloned into the yeast vector pPICZα.
Optimum expression of recombinant protein was obtained at 72 h induction in 1.5% methanol utilizing inoculum density (A600) of 80 and at pH-3.zero of the tradition medium. Identification of the purified protein was confirmed by mass spectrometry evaluation.
Additional research revealed the glycosylation sample and VLP nature of the purified protein. Immunization of BALB/c mice with these VLPs induced potent immune response as evidenced by the excessive ORF2 particular IgG titer and augmented splenocyte proliferation in a dose dependent method. 112-608aa ORF2 VLPs produced in P.
pastoris seems to be an appropriate candidate for improvement of diagnostic and prophylactic reagents towards the hepatitis E.

Environment friendly expression of enterovirus 71 based mostly on virus-like particles vaccine.

Enterovirus (EV) 71 is the principle pathogen related to hand-foot-mouth illness (HFMD) and may result in the illness with extreme mortality in kids. Since 2009, within the Republic of Korea, an outbreak of EV71 C4a an infection with neurologic involvement emerged, the place in HFMD involvement was recognized and central nervous system problems had been reported.
On this research, EV71 C4a virus-like particles (VLPs) produced by recombinant expertise had been generated in a baculovirus expression system.
To enhance the manufacturing yield, EV71 VLP was constructed utilizing the twin promoter system baculovirus P1 and 3CD (baculo-P1-3CD), which harbored each the structural protein-encoding P1 area beneath the management of the polyhedron promoter and the 3CD protease gene beneath the regulation of the CMV-IE, lef3, gp41, or chitinase promoters to reinforce the extent of gene transcription.
Environment friendly VLP expression was demonstrated via optimization of incubation time and bug cell kind. As well as, to guage the potential of VLP as a vaccine candidate, we examined the neutralizing antibodies and complete anti-EV71 IgG from the purified EV71 C4a VLP serum. The recombinant EV71 VLP exhibited the morphology of self-assembled VLP, as decided by electron microscopy.
Use of baculo-P1-3CD-gp41 led to a excessive yield (11.3mg/L < 40kDa) of VLPs in Excessive-FiveTM cells at Three days post-infection. Moreover, the potential of VLP as a vaccine was evaluated via the neutralizing capability elicited by the purified EV71 VLP after immunization of BALB/c mice, which was proven to induce potent and long-lasting humoral immune responses as evidenced by the cross-neutralization titer.
Our outcomes may very well be used to expedite the developmental course of for vaccines beneath medical trials and to make sure manufacturing consistency for licensing necessities.

Rift Valley fever virus structural and nonstructural proteins: recombinant protein expression and immunoreactivity towards antisera from sheep.

The Rift Valley fever virus (RVFV) encodes the structural proteins nucleoprotein (N), aminoterminal glycoprotein (Gn), carboxyterminal glycoprotein (Gc), and L protein, 78-kD, and the nonstructural proteins NSm and NSs.
Utilizing the baculovirus system, we expressed the full-length coding sequence of N, NSs, NSm, Gc, and the ectodomain of the coding sequence of the Gn glycoprotein derived from the virulent pressure of RVFV ZH548.
Western blot evaluation utilizing anti-His antibodies and monoclonal antibodies towards Gn and N confirmed expression of the recombinant proteins, and in vitro biochemical evaluation confirmed that the 2 glycoproteins, Gn and Gc, had been expressed in glycosylated type.
Immunoreactivity profiles of the recombinant proteins in western blot and in oblique enzyme-linked immunosorbent assay towards a panel of antisera obtained from vaccinated or wild kind (RVFV)-challenged sheep confirmed the outcomes obtained with anti-His antibodies and demonstrated the suitability of the baculo-expressed antigens for diagnostic assays.
As well as, these recombinant proteins may very well be precious for the event of diagnostic strategies that differentiate contaminated from vaccinated animals (DIVA).
Expression, Purification and Characterization of the Hepatitis E Virus Like-Particles in the Pichia pastoris.

Mrp2/Abcc2 transport exercise is stimulated by protein kinase Calpha in a baculo virus co-expression system.

Cholestatic and choleretic impact are well-known for protein kinase C activator and inhibitor, respectively. Nonetheless, post-translational regulation, particularly the impact of phosphorylation standing of the biliary transporters on their intrinsic transport exercise has not been totally understood.
On this research, impact of phosphorylation on the transport exercise of Mrp2, a biliary natural anion transporter, was examined in membrane vesicles remoted from Sf9 cells co-expressing extra quantity of protein kinase Calpha (PKCalpha).
Mrp2-mediated transport exercise was enhanced to three-fold by co-expressing PKCalpha. On the similar time, phosphorylation of Mrp2 was additionally detected. The Km and Vmax values for the transport of [3H]estradiol-17beta-D-glucuronide exhibited a 1.5-fold lower and a 1.9-fold enhance, respectively.
Probenecid (100 microM) and benzylpenicillin (1 mM), each are activator of Mrp2, didn’t stimulated the transport exercise of phosphorylated Mrp2. Alternatively, transport exercise was additional stimulated by Estron-3-sulfate and taurocholic acid.
Related mechanism that occurred within the presence of probenecid and benzylpenicillin, however completely different from that occurred within the presence of Estron-3-sulfate and taurocholic acid appears to be concerned within the stimulation.
Contemplating the discrepancy between the earlier in vivo inhibitory impact of PKC activators and our in vitro stimulatory impact of PKCalpha on Mrp2 transport exercise, direct modulation of Mrp2-transport exercise could also be minor if any beneath in vivo situation.

Synthesis of alpha-gal epitopes on influenza virus vaccines, by recombinant alpha 1,3galactosyltransferase, permits the formation of immune complexes with the pure anti-Gal antibody.

Synthesis of the carbohydrate construction Gal alpha 1-3Gal beta 1-4GlcNAc-R (termed the alpha-gal epitope) on viral glycoproteins is of curiosity due to the big quantities of pure antibody (anti-Gal) produced in people towards this epitope.
The presence of alpha-gal epitopes on inactivated virus or subviral vaccines is prone to improve vaccine immunogenicity via in vivo complexing with anti-Gal and the next focusing on of the vaccine to Fcy receptors on antigen presenting cells.
Our earlier research have demonstrated the elevated in vitro immunogenicity of inactivated influenza virus complexed with the anti-Gal antibody. Right here we exhibit a way for engineering the expression of alpha-gal epitopes on influenza virus hemagglutinin (HA) by recombinant alpha 1,3galactosyltransferase (r alpha 1,3GT).
We additional exhibit the formation of immune complexes between this de novo synthesized epitope and anti-Gal. HA has a number of N-acetyllactosamine buildings which function wonderful acceptors for r alpha 1,3GT.
The luminal portion of marmoset alpha 1,3GT cDNA was produced in giant quantities within the baculo virus system and remoted by affinity chromatography on nickel-Sepharose columns. r alpha 1,3GT successfully transferred galactose from UDP-Gal to the N-acetyllactosamine residues of HA on the intact virion or to remoted HA molecules.
A minimum of 3000 alpha-gal epitopes had been de novo synthesized per virion. The pure anti-Gal antibody certain to those epitopes in ELISA, in western blots and in answer, forming distinct immune complexes.

Platinum Retroviral Expression System, Amphotropic

VPK-301 1 kit
EUR 1169
Description: Retroviral vectors are useful for delivering genes of interest into a target cells where integration into the genome is desired. However, traditional retroviral expression technologies result in low viral titers, making gene expression studies challenging. Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection. This system includes our exclusive Platinum-A Packaging cell line, which contains the gag and pol genes and an amphotropic envelope protein for viral packaging. Simply clone your gene of interest into the vector provided and transfect into the packaging cells.

Platinum Retroviral Expression System, Pantropic

VPK-302 1 kit
EUR 1169
Description: Retroviral vectors are useful for delivering genes of interest into a target cells where integration into the genome is desired. However, traditional retroviral expression technologies result in low viral titers, making gene expression studies challenging. Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection. This system includes our exclusive Platinum-GP Packaging cell line which contains the gag and pol genes necessary for viral packaging. Simply clone your gene of interest into the vector provided and co-transfect into the packaging cells along with the included VSVG plasmid.

ViraSafe Universal Lentiviral Expression System, Ecotropic

VPK-211-ECO 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

ViraSafe Universal Lentiviral Expression System, Pantropic

VPK-211-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.

ViraSafe Lentiviral Expression System (Puro), Ecotropic

VPK-212-ECO 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

ViraSafe Lentiviral Expression System (Puro), Pantropic

VPK-212-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.

ViraSafe Lentiviral Expression System (Neo) Ecotropic

VPK-213-ECO 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

ViraSafe Lentiviral Expression System (Neo), Pantropic

VPK-213-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.

ViraSafe Lentiviral Expression System (Hygro), Ecotropic

VPK-214-ECO 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

ViraSafe Lentiviral Expression System (Hygro), Pantropic

VPK-214-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.

RAPAd® Universal Adenoviral Expression System

VPK-250 1 kit
EUR 1036
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed. The RAPAd® Universal Adenoviral Expression System (# VPK-250) contains a promoterless shuttle vector to allow you to insert the promoter of your choice along with your gene of interest to maximize gene expression.

RAPAd® RSV Adenoviral Expression System

VPK-251 1 kit
EUR 1036
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.

RAPAd® CMV Adenoviral Expression System

VPK-252 1 kit
EUR 1036
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.

RAPAd® miRNA Adenoviral Expression System

VPK-253 1 kit
EUR 1036
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.

Platinum HSC Retroviral Expression System, Ecotropic

VPK-306 1 kit
EUR 1169
Description: Retroviral vectors are useful for delivering genes of interest into a target cells where integration into the genome is desired. However, traditional retroviral expression technologies result in low viral titers, making gene expression studies challenging. Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection. This system includes our exclusive Platinum-E Packaging cell line, which contains the gag and pol genes and an ecotropic envelope protein for viral packaging. Simply clone your gene of interest into the vector provided and transfect into the packaging cells.

Platinum HSC Retroviral Expression System, Amphotropic

VPK-307 1 kit
EUR 1169
Description: Retroviral vectors are useful for delivering genes of interest into a target cells where integration into the genome is desired. However, traditional retroviral expression technologies result in low viral titers, making gene expression studies challenging. Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection. This system includes our exclusive Platinum-A Packaging cell line, which contains the gag and pol genes and an amphotropic envelope protein for viral packaging. Simply clone your gene of interest into the vector provided and transfect into the packaging cells.

Platinum HSC Retroviral Expression System, Pantropic

VPK-308 1 kit
EUR 1169
Description: Retroviral vectors are useful for delivering genes of interest into a target cells where integration into the genome is desired. However, traditional retroviral expression technologies result in low viral titers, making gene expression studies challenging. Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection. This system includes our exclusive Platinum-GP Packaging cell line which contains the gag and pol genes necessary for viral packaging. Simply clone your gene of interest into the vector provided and co-transfect into the packaging cells along with the included VSVG plasmid.

AAV-DJ Helper Free Expression System

VPK-410-DJ 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2.

AAV-1 Helper Free Expression System

VPK-410-SER1 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-2 Helper Free Expression System

VPK-410-SER2 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-3 Helper Free Expression System

VPK-410-SER3 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-4 Helper Free Expression System

VPK-410-SER4 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-5 Helper Free Expression System

VPK-410-SER5 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-6 Helper Free Expression System

VPK-410-SER6 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

scAAV-DJ Helper Free Expression System

VPK-430-DJ 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2.

scAAV-1 Helper Free Expression System

VPK-430-SER1 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

scAAV-2 Helper Free Expression System

VPK-430-SER2 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

scAAV-3 Helper Free Expression System

VPK-430-SER3 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

scAAV-4 Helper Free Expression System

VPK-430-SER4 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

scAAV-5 Helper Free Expression System

VPK-430-SER5 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

scAAV-6 Helper Free Expression System

VPK-430-SER6 1 kit
EUR 1239
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

ViraSafe Lentiviral Bicistronic Expression System (Puro), Ecotropic

VPK-215-ECO 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

ViraSafe Lentiviral Bicistronic Expression System (Puro), Pantropic

VPK-215-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.

ViraSafe Lentiviral Bicistronic Expression System (Neo), Ecotropic

VPK-216-ECO 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

ViraSafe Lentiviral Bicistronic Expression System (Neo), Pantropic

VPK-216-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.

ViraSafe Lentiviral Bicistronic Expression System (Hygro), Ecotropic

VPK-217-ECO 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

ViraSafe Lentiviral Bicistronic Expression System (Hygro), Pantropic

VPK-217-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.

ViraSafe Lentiviral Bicistronic Expression System (Blasticidin), Ecotropic

VPK-219-ECO 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

ViraSafe Lentiviral Bicistronic Expression System (Blasticidin), Pantropic

VPK-219-PAN 1 kit
EUR 1163
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.
These information recommend that in vivo administration of such vaccines will consequence of their complexing with anti-Gal, and thus might result in their elevated immunogenicity.

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