Fc Receptor Blocker

Product details

Applications

Flow cytometry (flow)

Functional test (FN)

Proven dilution: 20 µL / test

Product specifications

Species reactivity: Human

Published species: Human, mouse, zebrafish

Host/isotype: Does not apply

Class: Polyclonal

Writes: Antibody

Conjugate: Unconjugated

Form: Liquid

Concentration: 1 mg / mL

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2

Contains: 0.09% sodium azide

Storage conditions: 4 ° C

RRID: AB_468582

Product-specific information

Description: The human FcgammaR binding inhibitor can be used to inhibit the non-specific binding of the Fc-gamma receptor (FcgammaR) of mouse monoclonal antibodies used for flow cytometric analysis of human tissue. Four different classes of Fcgamma receptors have been identified: FcgammaRI (CD64), FcgammaRII (CD32), and FcgammaRIII (CD16), and are expressed at different levels in multiple cell lines, including high expression in myeloid, granulocytic, and B cells.

The biological function of FcgammaR, including initiation of endocytosis, phagocytosis, and antigen presentation, is triggered upon binding of host immunoglobulin. The degree to which mouse monoclonal antibodies will bind to human FcgammaR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will show different binding to human Fcgamma

Informed Applications: The purified Fc receptor binding inhibitor has been reported to be used in flow cytometric analysis. To inhibit non-specific binding of mouse monoclonal antibodies, add human Fc receptor binding inhibitor to samples and incubate on ice for 20 minutes. Without washing, proceed to stain with the primary antibody.

Tested Applications: The purified human Fc gamma binding inhibitor has been previously assessed and tested by inhibiting the binding of fluorochrome-conjugated isotype controls to U937 cells. This can be used at 20 µL per test. A test is defined as the amount (µg) of antibody that stains a cell sample in a final volume of 100 µL. The number of cells must be determined empirically, but can range from 10 ^ 5 to 10 ^ 8 cells/test.

Purity: greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-fabrication filtering.

Destination information

Four different classes of Fc receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and are expressed at varying levels in multiple cell lines, including high expression in myeloid, granulocytic, and B cells. Function Biological FcyR, which includes the initiation of endocytosis, phagocytosis, and antigen presentation, is triggered upon binding of host immunoglobulin. The degree to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will show different binding to human FcyRs.

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