
Hedgehog signaling in gastrointestinal carcinogenesis and the gastrointestinal tumor microenvironment
circEHBP1 promotes lymphangiogenesis and lymphatic metastasis of bladder most cancers by way of miR-130a-3p/TGFβR1/VEGF-D signaling
Concentrating on TGF-β-Mediated SMAD Signaling Pathway by way of Novel Recombinant Cytotoxin II: A Potent Protein from Naja naja oxiana Venom in Melanoma
Full-length IL-33 regulates Smad3 phosphorylation and gene transcription in a particular AP2-dependent method
Western blotting analyses revealed that FLIL33 overexpression elevated ranges, however didn’t have an effect on subcellular distribution, of the AP2A1 and AP2B1 subunits of the adaptor protein advanced 2 (AP2), a identified TGFBR binding companion. siRNA-mediated inhibition of those subunits blocked FLIL33-induced Smad3 phosphorylation, whereas AP2 subunit overexpression induced Smad3 phosphorylation even within the absence of FLIL33.
![]() Rat Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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DLR-Smad3-Ra-48T | DL Develop | 48T | EUR 549 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Mothers Against Decapentaplegic Homolog 3 (Smad3) in samples from tissue homogenates, cell lysates or other biological fluids. |
![]() Rat Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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DLR-Smad3-Ra-96T | DL Develop | 96T | EUR 718 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Mothers Against Decapentaplegic Homolog 3 (Smad3) in samples from tissue homogenates, cell lysates or other biological fluids. |
![]() Human Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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RDR-Smad3-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 544 |
![]() Human Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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RDR-Smad3-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 756 |
![]() Mouse Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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RDR-Smad3-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 557 |
![]() Mouse Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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RDR-Smad3-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 774 |
![]() Rat Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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RDR-Smad3-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 583 |
![]() Rat Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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RDR-Smad3-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 811 |
![]() Human Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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RD-Smad3-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 521 |
![]() Human Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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RD-Smad3-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 723 |
![]() Mouse Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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RD-Smad3-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 533 |
![]() Mouse Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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RD-Smad3-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 740 |
![]() Rat Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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RD-Smad3-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 557 |
![]() Rat Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit |
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RD-Smad3-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 775 |
![]() Smad3 antibody |
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70R-35222 | Fitzgerald | 100 ug | EUR 327 |
Description: Purified Rabbit polyclonal Smad3 antibody |
![]() Smad3 antibody |
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70R-35294 | Fitzgerald | 100 ug | EUR 327 |
Description: Purified Rabbit polyclonal Smad3 antibody |
![]() SMAD3 antibody |
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70R-35643 | Fitzgerald | 100 ug | EUR 349 |
Description: Rabbit polyclonal SMAD3 antibody |
RNA-Seq transcriptomic analyses revealed that fibroblast stimulation with TGF-β induced main adjustments in expression ranges of quite a few genes, whereas overexpression of FLIL33 induced modest expression adjustments in a small variety of genes. Moreover, qRT-PCR assessments demonstrated that regardless of inducing Smad3 phosphorylation, FLIL33 didn’t induce collagen gene transcription and even mildly attenuated TGF-β-induced ranges of collagen I and III mRNAs.
We conclude that FLIL33 induces Smad3 phosphorylation by means of a TGF-β-independent however TGF-β receptor- and AP2- dependent mechanism and has restricted downstream transcriptomic penalties.