DL-Serine Assay Kit

Properties

Use: sufficient for 100 fluorometric tests

Detection method: fluorometric

Storage temperature: −20 ° C

Related categories: Metabolism test kits

General description

Serine is one of the 20 natural amino acids used by all organisms in the biosynthesis of proteins. Having a single chiral centre, serine can exist as either of two stereoisomers (L-Serine and D-Serine). In addition to its function as a building block for proteins, L-Serine serves as a precursor for many vital biomolecules, such as phosphatidylserine, sphingomyelin, and the amino acids glycine and cysteine.

The non-proteogenic isomer D-Serine is also synthesized from L-Serine in the mammalian brain. While most D-amino acids are only present in small amounts in mammals, D-Serine is a vital neurotransmitter that acts as a coagonist for N-methyl-D-aspartate (NMDA) -type synaptic ion channels activated by glutamate. Altered D-serine metabolism can contribute to various neuropsychiatric disorders, including depression, schizophrenia, epilepsy, and dementia.

Features and Benefits

Compatible with high-performance handling systems.

Suitability

Suitable for the estimation of D- and L-serine concentrations in human or animal biological fluids (plasma, serum, CSF, etc.), soft tissue homogenates (i.e., liver, brain, etc.) and lysates of cultured cells (adherent cells or in suspension).

Beginning

The DL-Serine Assay Kit allows the quantification of L- and D-Serine in biological fluids and tissues. The assay is based on the conversion of L-Serine to D-Serine, which is metabolized to an intermediate product that is subsequently oxidized and reacted with a probe to form a stable fluorophore (Ex / Em = 535/587 nm). Samples can be divided and analyzed simultaneously for the quantification of both D-Serine and total DL-Serine. The assay is unaffected by physiological concentrations of other amino acids, is high throughput adaptive, and can detect less than 1 µM of serine.

Safety Information

Flashpoint (F): 188.6 ° F

Flashpoint (C): 87 ° C

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