Rv3634c from Mycobacterium tuberculosis H37Rv encodes an enzyme with UDP-Gal/Glc and UDP-GalNAc 4-epimerase activities.

Rv3634c from Mycobacterium tuberculosis H37Rv encodes an enzyme with UDP-Gal/Glc and UDP-GalNAc 4-epimerase activities.

A bioinformatics research revealed that Mycobacterium tuberculosis H37Rv (Mtb) incorporates sequence homologs of Campylobacter jejuni protein glycosylation enzymes. The ORF Rv3634c from Mtb was recognized as a sequence homolog of C. jejuni UDP-Gal/GalNAc 4-epimerase. This research reviews the cloning of Rv3634c and its expression as an N-terminal His-tagged protein. The recombinant protein was proven to have UDP-Gal/Glc 4-epimerase exercise by GOD-POD assay and by reverse section HPLC. This enzyme was proven to have UDP-GalNAc 4-epimerase exercise additionally. Residues Ser121, Tyr146 and Lys150 have been proven by site-directed mutagenesis to be vital for enzyme exercise.

Mutation of Ser121 and Tyr146 to Ala and Phe, respectively, led to finish lack of exercise whereas mutation of Lys150 to Arg led to partial lack of exercise. There have been no gross modifications within the secondary constructions of any of those three mutants. These outcomes counsel that Ser121 and Tyr146 are important for epimerase exercise of Rv3634c. UDP-Gal/Glc 4-epimerases from different organisms even have a catalytic triad consisting of Ser, Tyr and Lys.

The triad carries out proton switch from nucleotide sugar to NAD+ and again, thus effecting the epimerization of the substrate. Addition of NAD+ to Lys150 considerably abrogates the lack of exercise, suggesting that, as in different epimerases, NAD+ is related to Rv3634c. Right here, we present that EIIA(Glc) of the glucose-specific PTS system can be required for the traditional decay of those sRNAs and that it acts by binding to the EAL area of CsrD. Solely the unphosphorylated type of EIIA(Glc) sure to CsrD in vitro and was able to activating CsrB/C turnover in vivo.

Csr is a conserved international regulatory system, which makes use of the sequence-specific RNA-binding protein CsrA to activate or repress gene expression by binding to mRNA and altering translation, stability and/or transcript elongation. In Escherichia coli, CsrA exercise is regulated by two sRNAs, CsrB and CsrC, which bind to a number of CsrA dimers, thereby sequestering this protein away from its mRNA targets. Turnover of CsrB/C sRNAs is tightly regulated by a GGDEF-EAL area protein, CsrD, which targets them for cleavage by RNase E.

The conundrum of UDP-Glc entrance into the yeast ER lumen.

UDP-Glc entrance into the endoplasmic reticulum (ER) of eukaryotic cells is a key step within the high quality management of glycoprotein folding, a mechanism requiring switch of a Glc residue from the nucleotide sugar (NS) to glycoprotein folding intermediates by the UDP-Glc:glycoprotein glucosyltransferase (UGGT). In keeping with a bioinformatics search there are solely eight genes within the Schizosaccharomyces pombe genome belonging to the three Pfam households to which all recognized nucleotide-sugar transporters (NSTs) of the secretory pathway belong.

The protein merchandise of two of them (hut1+ and yea4+) localize to the ER, these of genes gms1+, vrg4+, pet1+, pet2+ and pet3+ to the Golgi, whereas that of gms2+ has an unknown location. Right here we show that (1) Δhut1 and Δgpt1 (UGGT null) mutants share a number of phenotypic options; (2) Δhut1 mutants present a 50% discount in UDP-Glc transport into ER-derived membranes; (3) in vivo UDP-Glc ER entrance occurred in Δhut1Δyea4Δgms2 mutants and in cells through which Δhut1 disruption was mixed with that of every of 4 of the genes encoding Golgi-located proteins.

Rv3634c from Mycobacterium tuberculosis H37Rv encodes an enzyme with UDP-Gal/Glc and UDP-GalNAc 4-epimerase activities.

Subsequently, disruption of all genes whose merchandise localize to the ER or have an unknown location didn’t obliterate UDP-Glc ER entrance. We conclude that the hut1+ gene product is concerned in UDP-Glc entrance into the ER, however that a minimum of one other as but unknown NST displaying an unconventional sequence operates within the yeast secretory pathway. This conclusion agrees with our earlier outcomes exhibiting that UDP-Glc entrance into the yeast ER doesn’t observe the classical NST antiport mechanism.

The research on the preparation and characterization of gene-loaded immunomagnetic albumin nanospheres and their anti-cell proliferative impact mixed with magnetic fluid hyperthermia on GLC-82 cells.

As one of the vital frequent malignant tumors, the medical and socio-economic penalties of lung most cancers are important. At the moment, surgical procedure is the primary therapy technique for this illness, however the survival charges of lung most cancers sufferers are usually not ultimate as a result of excessive recurrence fee of the illness. Subsequently, many researchers are exploring new particular therapeutic strategies which might be extremely healing and minimally cytotoxic to wholesome tissues. To this finish, albumin nanospheres concurrently have been loaded with super-paramagnetic iron oxide nanoparticles (as gene vector and anticancer gene), and plasmid pDONR223-IFNG, and modified with anti-EGFR monoclonal antibody cetuximab as remedy.

Focusing on brokers, specifically gene-loaded immunomagnetic albumin nanospheres (cetuximab [C225]-IFNG-IMANS), have been ready for focused lung carcinoma cells (GLC-82 cell strains). Transmission electron microscopy photographs confirmed that the C225-IFNG-IMANS have been efficiently ready, and the power of the nanospheres to focus on GLC-82 cells in vitro was confirmed by Prussian blue staining, immunofluorescence experiments, and magnetic resonance imaging. Transfection images and agarose gel electrophoresis proved that pDONR223-IFNG might be encased within the albumin nanospheres.

A Cell Counting Equipment-Eight assay confirmed that the mixture remedy group had considerably extra therapeutic results on GLC-82 cells than different remedy teams. A stream cytometry assay confirmed that the apoptotic index of the mixed therapy group was 67.68%, whereas the indices of the C225 group, gene remedy group, and magnetic fluid hyperthermia group have been 12.2%, 16.34%, and 20.04% respectively.

Genorise® Universal DNA Mini Kit 100

GR101076-100 100
EUR 357

Genorise® Universal DNA Mini Kit 200

GR101077 200
EUR 567

Genorise® Universal DNA Mini Kit 500

GR101077-500 500
EUR 1272

Methylamp Universal Methylated DNA Kit 

P-1011
  • EUR 684.66
  • EUR 292.60
  • EUR 442.20
  • 20 Modifications
  • 10 Modifications
  • 20 Modifications

VAHTS Universal DNA Library Prep Kit for MGI

NDM607-01 24 rxn
EUR 879.6

VAHTS Universal DNA Library Prep Kit for MGI

NDM607-02 96 rxn
EUR 2563.2

VAHTS Universal DNA library Prep Kit for MGI V4

NDM610-01 24 rxns
EUR 235.2

VAHTS Universal DNA library Prep Kit for MGI V4

NDM610-02 96 rxns
EUR 880

VAHTS Universal DNA Library Prep Kit for Illumina V3

ND607-01 24 rxn
EUR 529.2

VAHTS Universal DNA Library Prep Kit for Illumina V3

ND607-02 96 rxn
EUR 1686

VAHTS Universal DNA Library Prep Kit for Ion Torrent

ND701-01 24 rxn
EUR 879.6

VAHTS Universal DNA Library Prep Kit for Ion Torrent

ND701-02 96 rxn
EUR 2563.2

VAHTS Universal DNA Library Prep Kit for Illumina V4

ND610-01 24 rxns
EUR 235.2

VAHTS Universal DNA Library Prep Kit for Illumina V4

ND610-02 96 rxns
EUR 880

Methylamp Universal Methylated DNA Preparation Kit

P-1019 40 µg
EUR 416.7

EpiMag High Throughput DNA Isolation Universal Kit

P-1022-96 96 Samples
EUR 227.7

VAHTS Universal DNA Library Prep Kit for Ion Torrent V2

ND702-01 24 rxns
EUR 235.2

VAHTS Universal DNA Library Prep Kit for Ion Torrent V2

ND702-02 96 rxns
EUR 880

VAHTS Universal Pro DNA Library Prep Kit for MGI

NDM608-01 24 rxn
EUR 907.2

VAHTS Universal Pro DNA Library Prep Kit for MGI

NDM608-02 96 rxn
EUR 2740.8

VAHTS Universal Plus DNA Library Prep Kit for MGI

NDM617-01 24 rxn
EUR 988.8

VAHTS Universal Plus DNA Library Prep Kit for MGI

NDM617-02 96 rxn
EUR 2889.6

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

NDM627-01 24 rxns
EUR 250

VAHTS Universal Plus DNA Library Prep Kit for MGI V2

NDM627-02 96 rxns
EUR 920

VAHTS Universal Pro DNA Library Prep Kit for Illumina

ND608-01 24 rxn
EUR 558

VAHTS Universal Pro DNA Library Prep Kit for Illumina

ND608-02 96 rxn
EUR 1858.8

VAHTS Universal Plus DNA Library Prep Kit for Illumina

ND617-01 24 rxn
EUR 590.4

VAHTS Universal Plus DNA Library Prep Kit for Illumina

ND617-02 96 rxn
EUR 1926

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)

ND607-03 24 rxn
EUR 514.8

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)

ND607-04 96 rxn
EUR 1624.8

Universal platform

INC1112 EACH
EUR 1434

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

ND627-01 24 rxns
EUR 250

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

ND627-02 96 rxns
EUR 920

Universal Attachment

MIX1320 EACH
EUR 286.8

Universal V1-Puck

M-CP-111-021 1 PUCK
EUR 350
Description: Universal V1-Puck

Universal Oven UN30

OVE8314 EACH
EUR 1672.8

Universal Oven UN55

OVE8316 EACH
EUR 1952.4

Universal Oven UN75

OVE8318 EACH
EUR 2028

Universal Oven UF30

OVE8332 EACH
EUR 1911.6

Universal Oven UF55

OVE8334 EACH
EUR 2306.4

Universal Oven UF75

OVE8336 EACH
EUR 2433.6

UNIVERSAL BEER AGAR

U21-100-10kg 10 kg
EUR 1828.8

UNIVERSAL BEER AGAR

U21-100-2kg 2kg
EUR 444

UNIVERSAL BEER AGAR

U21-100-500g 500 g
EUR 164.4

Grant Universal Tray

BAT4622 EACH
EUR 314.4

Universal AC adapter

PIP3214 EACH
EUR 78

Universal Oven UN110

OVE8320 EACH
EUR 2598

Universal Oven UN160

OVE8322 EACH
EUR 3133.2

Universal Oven UN260

OVE8324 EACH
EUR 4148.4

Universal Oven UN450

OVE8326 EACH
EUR 5490

Universal Oven UN750

OVE8328 EACH
EUR 7356

Universal Oven UF110

OVE8338 EACH
EUR 2876.4

Universal Oven UF160

OVE8340 EACH
EUR 3740.4

Universal Oven UF260

OVE8342 EACH
EUR 4768.8

Universal Oven UF450

OVE8344 EACH
EUR 6244.8

Universal Oven UF750

OVE8346 EACH
EUR 8275.2

Universal Spring Tray

BAT7177 EACH
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Universal Oven UF1060

OVE8348 EACH
EUR 15282

NBS Universal Platform

SHA4050 EACH
EUR 1459.2

NBS Universal Platform

SHA4070 EACH
EUR 1059.6

NBS Universal Platform

SHA4232 EACH
EUR 580.8

NBS Universal Platform

SHA4256 EACH
EUR 711.6

NBS Universal Platform

SHA4272 EACH
EUR 1129.2

NBS Universal Platform

SHA4290 EACH
EUR 1519.2

Universal qPCR Mixture

QPCR1000-UNIV 4X1.25ml
EUR 331.44

Mains adaptor (universal)

ARG1248 EACH
EUR 135.78

Universal Oven UN30plus

OVE8350 EACH
EUR 2390.4

Universal Oven UN55plus

OVE8352 EACH
EUR 2608.8

Universal Oven UN75plus

OVE8354 EACH
EUR 2902.8

Universal Oven UF30plus

OVE8366 EACH
EUR 2523.6

Universal Oven UF55plus

OVE8368 EACH
EUR 2752.8

Universal Oven UF75plus

OVE8370 EACH
EUR 3300

Loopster Universal Disc

MIX1080 EACH
EUR 180

Universal Oven UN110plus

OVE8356 EACH
EUR 3327.6

Universal Oven UN260plus

OVE8358 EACH
EUR 4956

Universal Oven UN450plus

OVE8360 EACH
EUR 6390

Universal Oven UN750plus

OVE8362 EACH
EUR 8228.4

Universal Oven UF110plus

OVE8372 EACH
EUR 3807.6

Universal Oven UF160plus

OVE8374 EACH
EUR 4743.6

Universal Oven UF260plus

OVE8376 EACH
EUR 5660.4

Universal Oven UF450plus

OVE8378 EACH
EUR 7177.2

Universal Oven UF750plus

OVE8380 EACH
EUR 9790.8

IKA Universal Attachment

SHA2016 EACH
EUR 997.2

Universal attachment IKA

SHA2210 EACH
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Universal Ohaus Plug GB

46001779 EACH
EUR 16.8

Universal Stratus Adapter

MIC6036 EACH
EUR 592.8

Universal Oven UF1060plus

OVE8382 EACH
EUR 16224

Universal Spring Platform

MIX7184 EACH
EUR 885.6

Universal Spring Platform

H1000-P-SP 1 PC
EUR 640.9

Universal Spring Platform

H1010-P-SP 1 each
EUR 677.5

Universal tray for LSB12

BAT1062 EACH
EUR 300

Universal tray for LSB18

BAT1064 EACH
EUR 314.4

Azo Universal spray 500ml

CLE1996 EACH
EUR 10.29

2 Universal Clip Plates

MIX5190 EACH
EUR 110.4

18mmMag Universal ScrewCap

CHR3460 PK1000
EUR 1339.2

Universal (Aluminum) Platform

MIX1322 EACH
EUR 139.2

Universal Multichannel Stand

F161417 EACH
EUR 33.6

Universal Indicator Solution

UN10025 2.5L
EUR 205.2

Universal Indicator Solution

UN1005 50ML
EUR 21.31

Universal Indicator Solution

UN101 100ML
EUR 22.8

Universal Indicator Solution

UN1025 25L
EUR 812.08

Universal Indicator Solution

UN105 500ML
EUR 111.6

Universal Indicator Solution

UNB1010 10L
EUR 782.05

PCR plate-foil universal

A28216 1x100 pcs
EUR 112.8

PCR plate-foil universal

A28216L 5x100 pcs
EUR 372

AED Universal Wall Bracket

SAF1128 EACH
EUR 107.06

Wire Rack 2x 6 Universal

STA20020 EACH
EUR 36

Universal Detachment Solution

AD002 100 ml
EUR 177.6

UNIVERSAL PREENRICHMENT BROTH

U21-101-10kg 10 kg
EUR 898.8

UNIVERSAL PREENRICHMENT BROTH

U21-101-2Kg 2 Kg
EUR 242.4

UNIVERSAL PREENRICHMENT BROTH

U21-101-500g 500 g
EUR 109.2

5min Universal Ligation Mix

C311-01 50 rxn
EUR 256.8

5min Universal Ligation Mix

C311-02 100 rxn
EUR 339.6

18mm Mag Universal ScrewCap

CHR3466 PK1000
EUR 1266

18mm Mag Universal ScrewCap

CHR3468 PK1000
EUR 954

Pack of 10 universal seals

WAT4870 PK10
EUR 214.12

Universal Full Growth Medium

TM001 500 ml
EUR 403.2

Universal V1-Puck; Qnty 7

M-CP-111-021-7 7 PUCKS
EUR 2420
Description: Universal V1-Puck; Qnty 7

10 × Universal Primer Mix (UPM)

RA102-01 100 rxns (5 μl/rxn)
EUR 58

Universal Neutralization Buffer

NB001 100 ml
EUR 162

Glass universal 30ml sep cap

127030CS PK500
EUR 246

Universal Indicator pH 1-14

RRSP4455-D 500ml
EUR 8.63

Universal Indicator pH 1-14

RRSP4455-E 1L
EUR 15.86

Universal Indicator pH 1-14

RRSP4455-F 2.5L
EUR 30.22

Finntip 250 Universal 0.5-250ul 1000 Per bag

PIP3612 PK1000
EUR 94.8

Universal Platform Innova S44i

SHA3072 EACH
EUR 1449.6

Nunc Universal Lid PS Sterile

250002 PK60
EUR 294

Universal Platform 28 X 33cm

SHA6028 EACH
EUR 223.2

Universal Platform 33 X 33cm

SHA6032 EACH
EUR 319.2

Universal Platform 46 X 46cm

SHA6034 EACH
EUR 382.8

Universal Platform 46 X 61cm

SHA6036 EACH
EUR 430.8

Universal Platform 61 X 61cm

SHA6038 EACH
EUR 1034.4

Universal Platform 61 X 91cm

SHA6062 EACH
EUR 1310.4

A5 Boots Vinyl sole UNIVERSAL

SAF4750 100PR
EUR 655.41

A5 Boots Vinyl foot UNIVERSAL

SAF4756 100PR
EUR 825.14

Universal TT epitope P2 (830-844)

5-02048 4 x 1mg Ask for price

IKA AS4000.1 Universal Attachment

SHA2612 EACH
EUR 1251.6

SLS Pipette Tip Universal 1-200ul Loose

PIP7812 PK1000
EUR 26.63

50UL UNIVERSAL FIT FILTER TIPS

TF-50 1000/pk
EUR 501.6
Description: Filter Tip; Filter Tips Pipette - Axygen

Universal Casein Diluent/Blocker

85R-108 250 ml
EUR 276
Description: Ready-to-use biotin free reagent applicable for blocking ELISA plates

Universal Active Charcoal Filter

FUM1146 EACH
EUR 637.2

UNIVERSAL LINEAR 4 PLACE STAND

AP-USD4 1/pk
EUR 84
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

Sterile universal 30ml no label

128A/IRR PK400
EUR 196.8

HotTaq Probe qPCR Universal Mix

BT11004 250rxn
EUR 147.6
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Universal scissors length 150 mm

DD76147 EACH
EUR 36

10 x Universal Blocking Buffer

GR103005 200 mL
EUR 129

30ml Universal PS Printed Label

SLE7502 PK400
EUR 116.4

100UL UNIVERSAL FIT FILTER TIPS

TF-100 1000/pk
EUR 501.6
Description: Filter Tip; Filter Tips Pipette - Axygen

1000UL UNIVERSAL FIT FILTER TIPS

TF-1000 1000/pk
EUR 278.4
Description: Filter Tip; Filter Tips Pipette - Axygen

200UL UNIVERSAL FIT FILTER TIPS

TF-200 1000/pk
EUR 501.6
Description: Filter Tip; Filter Tips Pipette - Axygen

300UL UNIVERSAL FIT FILTER TIPS

TF-350 1000/pk
EUR 501.6
Description: Filter Tip; Filter Tips Pipette - Axygen

Universal Pipette Holder Assorted

PIP0164 EACH
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Universal Attachment for SHA20702

SHA2074 EACH
EUR 2494.8

Grant Universal platform 270x195mm

SHA7932 EACH
EUR 176.4

Universal Lids N Ste w/oRings

PCR0380 PK100
EUR 226.8

Finnpipette F3 1-10ul Universal

PIP0101 EACH
EUR 427.2

Finnpipette F3 2-20ul Universal

PIP0103 EACH
EUR 427.2

Universal Pipette Stand Clear 4

PIP0386 EACH
EUR 63.6

Universal Pipette Stand Clear 6

PIP0396 EACH
EUR 88.8

Platform universal flat-bed 4

SHA7952 EACH
EUR 638.4

Universal RNA - Rat Normal Tissues

R4434567 4x100 ug
EUR 635

Universal RNA - Rat Normal Tissues

R4434567-1 2x100 ug
EUR 347

Universal RNA - Dog Normal Tissues

R4734565 4x100 ug
EUR 766

Universal RNA - Dog Normal Tissues

R4734565-1 2x100 ug
EUR 450

Universal tiered rack blue colour

DD64012 EACH
EUR 37.2

Universal 3' miRNA Reverse Primer

MPH00000 150 ul / 10 uM
EUR 84

Universal Pipette Stand Orange 4

PIP0384 EACH
EUR 63.6

Ohaus Support Stand Universal-H

STI2048 EACH
EUR 308.4

Universal control tissue microarray

CTRL141 each
EUR 54
Description: Universal control tissue microarray, 7 types of Immune System normal tissues, 7 cases/14 cores

Nunc Universal Lid PS Non Sterile

250003 PK60
EUR 260.4

Universal miRNA qPCR Primer (10 µM)

abx298006-200ul 200 ul
EUR 226.8

Select 30ml Universal PS No Label

SLE7500 PK400
EUR 96

HotTaq EvaGreen qPCR Universal Mix

BT11104 250rxn
EUR 165.6
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Universal tiered rack green colour

DD64013 EACH
EUR 37.2

Adapter plates for Universal rotor

E5910753004 PK2
EUR 296.4

Universal RNA - Human Normal Tissues

R4234565 4x100 ug
EUR 766

Universal RNA - Human Normal Tissues

R4234565-1 2x100 ug
EUR 450

Universal RNA - Mouse Normal Tissues

R4334566 4x100 ug
EUR 635

Universal RNA - Mouse Normal Tissues

R4334566-1 2x100 ug
EUR 347

Memmert 53L Natural Universal Oven

UN55 EACH
EUR 1917.6

Finntip 250 Universal 0.5-250ul Yellow 500 Per box

PIP3614 PK500
EUR 50.4

Sterile DNase/RNase-free Universal Pipette Tip

AI200R1 200μL Ask for price

Universal 30ml ps Iw double bagged

128DB/IRR PK150
EUR 711.6

Bottle Universal 28ml Glass+Al Cap

BOT5006 PK144
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AceQ Universal Probe Master Mix V2

Q513-EN02 500 rxns (20 μl/rxn)
EUR 130.11

AceQ Universal Probe Master Mix V2

Q513-EN03 2,500 rxns (20 μl/rxn)
EUR 579.6

Universal tiered rack yellow colour

DD64014 EACH
EUR 37.2

Magic Clamp Universal Platform Mini

SHA1302 EACH
EUR 384

Universal RNA - Monkey Normal Tissues

R4534565 4x100 ug
EUR 766

Universal RNA - Monkey Normal Tissues

R4534565-1 2x100 ug
EUR 450

PPR Universal 30ml 128A/P No label

CON8000 PK400
EUR 160.8

Universal Indicator Strips pH 0-14

PAP1206 PK100
EUR 12

AceQ Universal SYBR qPCR Master Mix

Q511-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ Universal SYBR qPCR Master Mix

Q511-03 2500 rxn (20 μl/rxn)
EUR 775.2

Hettich Centrifuge Universal 320 Small Volume

CEN0401 EACH
EUR 5070

Universal RNA - Chicken Normal Tissues

R4C34565 4x100 ug
EUR 766

Universal RNA - Chicken Normal Tissues

R4C34565-1 2x100 ug
EUR 450

PhotoFlex rchrge batt + universal plug

WAT5108 EACH
EUR 288.54

RD 1 Universal mat for IKA rockers

MIX4240 EACH
EUR 32.4

Finntip 250 Universal 0.5-250ul Yellow 10 x 96 rack

PIP3668 PK960
EUR 121.2

Subsequently, the mixture of thermal therapy, molecular focused therapy, and gene therapy synergistically targets GLC-82 cells, and the usage of C225-IFNG-IMANS as a gene or drug provider provides a novel and promising strategy for the therapy of lung most cancers.

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