Rv3634c from Mycobacterium tuberculosis H37Rv encodes an enzyme with UDP-Gal/Glc and UDP-GalNAc 4-epimerase activities.

Rv3634c from Mycobacterium tuberculosis H37Rv encodes an enzyme with UDP-Gal/Glc and UDP-GalNAc 4-epimerase activities.

A bioinformatics research revealed that Mycobacterium tuberculosis H37Rv (Mtb) incorporates sequence homologs of Campylobacter jejuni protein glycosylation enzymes. The ORF Rv3634c from Mtb was recognized as a sequence homolog of C. jejuni UDP-Gal/GalNAc 4-epimerase. This research reviews the cloning of Rv3634c and its expression as an N-terminal His-tagged protein. The recombinant protein was proven to have UDP-Gal/Glc 4-epimerase exercise by GOD-POD assay and by reverse section HPLC. This enzyme was proven to have UDP-GalNAc 4-epimerase exercise additionally. Residues Ser121, Tyr146 and Lys150 have been proven by site-directed mutagenesis to be vital for enzyme exercise.

Mutation of Ser121 and Tyr146 to Ala and Phe, respectively, led to finish lack of exercise whereas mutation of Lys150 to Arg led to partial lack of exercise. There have been no gross modifications within the secondary constructions of any of those three mutants. These outcomes counsel that Ser121 and Tyr146 are important for epimerase exercise of Rv3634c. UDP-Gal/Glc 4-epimerases from different organisms even have a catalytic triad consisting of Ser, Tyr and Lys.

The triad carries out proton switch from nucleotide sugar to NAD+ and again, thus effecting the epimerization of the substrate. Addition of NAD+ to Lys150 considerably abrogates the lack of exercise, suggesting that, as in different epimerases, NAD+ is related to Rv3634c. Right here, we present that EIIA(Glc) of the glucose-specific PTS system can be required for the traditional decay of those sRNAs and that it acts by binding to the EAL area of CsrD. Solely the unphosphorylated type of EIIA(Glc) sure to CsrD in vitro and was able to activating CsrB/C turnover in vivo.

Csr is a conserved international regulatory system, which makes use of the sequence-specific RNA-binding protein CsrA to activate or repress gene expression by binding to mRNA and altering translation, stability and/or transcript elongation. In Escherichia coli, CsrA exercise is regulated by two sRNAs, CsrB and CsrC, which bind to a number of CsrA dimers, thereby sequestering this protein away from its mRNA targets. Turnover of CsrB/C sRNAs is tightly regulated by a GGDEF-EAL area protein, CsrD, which targets them for cleavage by RNase E.

The conundrum of UDP-Glc entrance into the yeast ER lumen.

UDP-Glc entrance into the endoplasmic reticulum (ER) of eukaryotic cells is a key step within the high quality management of glycoprotein folding, a mechanism requiring switch of a Glc residue from the nucleotide sugar (NS) to glycoprotein folding intermediates by the UDP-Glc:glycoprotein glucosyltransferase (UGGT). In keeping with a bioinformatics search there are solely eight genes within the Schizosaccharomyces pombe genome belonging to the three Pfam households to which all recognized nucleotide-sugar transporters (NSTs) of the secretory pathway belong.

The protein merchandise of two of them (hut1+ and yea4+) localize to the ER, these of genes gms1+, vrg4+, pet1+, pet2+ and pet3+ to the Golgi, whereas that of gms2+ has an unknown location. Right here we show that (1) Δhut1 and Δgpt1 (UGGT null) mutants share a number of phenotypic options; (2) Δhut1 mutants present a 50% discount in UDP-Glc transport into ER-derived membranes; (3) in vivo UDP-Glc ER entrance occurred in Δhut1Δyea4Δgms2 mutants and in cells through which Δhut1 disruption was mixed with that of every of 4 of the genes encoding Golgi-located proteins.

Rv3634c from Mycobacterium tuberculosis H37Rv encodes an enzyme with UDP-Gal/Glc and UDP-GalNAc 4-epimerase activities.

Subsequently, disruption of all genes whose merchandise localize to the ER or have an unknown location didn’t obliterate UDP-Glc ER entrance. We conclude that the hut1+ gene product is concerned in UDP-Glc entrance into the ER, however that a minimum of one other as but unknown NST displaying an unconventional sequence operates within the yeast secretory pathway. This conclusion agrees with our earlier outcomes exhibiting that UDP-Glc entrance into the yeast ER doesn’t observe the classical NST antiport mechanism.

The research on the preparation and characterization of gene-loaded immunomagnetic albumin nanospheres and their anti-cell proliferative impact mixed with magnetic fluid hyperthermia on GLC-82 cells.

As one of the vital frequent malignant tumors, the medical and socio-economic penalties of lung most cancers are important. At the moment, surgical procedure is the primary therapy technique for this illness, however the survival charges of lung most cancers sufferers are usually not ultimate as a result of excessive recurrence fee of the illness. Subsequently, many researchers are exploring new particular therapeutic strategies which might be extremely healing and minimally cytotoxic to wholesome tissues. To this finish, albumin nanospheres concurrently have been loaded with super-paramagnetic iron oxide nanoparticles (as gene vector and anticancer gene), and plasmid pDONR223-IFNG, and modified with anti-EGFR monoclonal antibody cetuximab as remedy.

Focusing on brokers, specifically gene-loaded immunomagnetic albumin nanospheres (cetuximab [C225]-IFNG-IMANS), have been ready for focused lung carcinoma cells (GLC-82 cell strains). Transmission electron microscopy photographs confirmed that the C225-IFNG-IMANS have been efficiently ready, and the power of the nanospheres to focus on GLC-82 cells in vitro was confirmed by Prussian blue staining, immunofluorescence experiments, and magnetic resonance imaging. Transfection images and agarose gel electrophoresis proved that pDONR223-IFNG might be encased within the albumin nanospheres.

A Cell Counting Equipment-Eight assay confirmed that the mixture remedy group had considerably extra therapeutic results on GLC-82 cells than different remedy teams. A stream cytometry assay confirmed that the apoptotic index of the mixed therapy group was 67.68%, whereas the indices of the C225 group, gene remedy group, and magnetic fluid hyperthermia group have been 12.2%, 16.34%, and 20.04% respectively.

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 84
Description: Bioscience Mol Bio; DNA Ladder

QPCR Kit DNA Universal f Mmix

MOL6608 EACH
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QPCR Kit DNA Universal m Mmix

MOL6612 EACH
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Methylamp Universal Methylated DNA Preparation Kit

P-1019 40 µg
EUR 416.7

Genorise® Universal DNA Mini Kit 50

GR101076 50
EUR 267.6

Genorise® Universal DNA Mini Kit 100

GR101076-100 100
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Genorise® Universal DNA Mini Kit 200

GR101077 200
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Genorise® Universal DNA Mini Kit 500

GR101077-500 500
EUR 1526.4

EpiMag High Throughput DNA Isolation Universal Kit

P-1022-96 96 Samples
EUR 227.7

2K DNA Marker

20-abx098045
  • EUR 309.60
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  • 2.5 ml
  • 500 ul

5K DNA Marker

20-abx098048
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  • 2.5 ml
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15K DNA Marker

20-abx098049
  • EUR 326.40
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  • 2.5 ml
  • 500 ul

1Kb DNA Marker

20-abx098050
  • EUR 309.60
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  • 2.5 ml
  • 500 ul

100bp DNA Marker

20-abx098052
  • EUR 326.40
  • EUR 226.80
  • 2.5 ml
  • 500 ul

100bp DNA Marker

D1100-050 50㎍
EUR 198

100bp DNA Marker

D1100-250 5X50㎍
EUR 660

1Kb DNA Marker

D1101-050 50㎍
EUR 157.2

1Kb DNA Marker

D1101-250 5X50㎍
EUR 360

DL5000 DNA Marker

MD102-01 250 μl
EUR 128.4

DL5000 DNA Marker

MD102-02 500 μl
EUR 135.6

DL15000 DNA Marker

MD103-01 250 μl
EUR 130.8

DL15000 DNA Marker

MD103-02 500 μl
EUR 138

VAHTS Universal DNA Library Prep Kit for MGI

NDM607-01 24 rxn
EUR 879.6

VAHTS Universal DNA Library Prep Kit for MGI

NDM607-02 96 rxn
EUR 2563.2

1Kb Plus DNA Marker

20-abx098051
  • EUR 360.00
  • EUR 243.60
  • 2.5 ml
  • 500 ul

DL2000 Plus DNA Marker

MD101-01 250 μl
EUR 128.4

DL2000 Plus DNA Marker

MD101-02 500 μl
EUR 135.6

Linear Kinetoplast DNA Marker

TG2018-1 10 ug
EUR 214.8

Decatenated Kinetoplast DNA Marker

TG2020-1 10 ug
EUR 228

Relaxed pRYG DNA Marker

TG2025-1 10 ug
EUR 321.6

Linear pRYG DNA Marker

TG2028-1 10 ug
EUR 214.8

VAHTS Universal DNA Library Prep Kit for Illumina V3

ND607-01 24 rxn
EUR 529.2

VAHTS Universal DNA Library Prep Kit for Illumina V3

ND607-02 96 rxn
EUR 1686

VAHTS Universal Pro DNA Library Prep Kit for Illumina

ND608-01 24 rxn
EUR 558

VAHTS Universal Pro DNA Library Prep Kit for Illumina

ND608-02 96 rxn
EUR 1858.8

VAHTS Universal Plus DNA Library Prep Kit for Illumina

ND617-01 24 rxn
EUR 590.4

VAHTS Universal Plus DNA Library Prep Kit for Illumina

ND617-02 96 rxn
EUR 1926

VAHTS Universal DNA Library Prep Kit for Ion Torrent

ND701-01 24 rxn
EUR 879.6

VAHTS Universal DNA Library Prep Kit for Ion Torrent

ND701-02 96 rxn
EUR 2563.2

VAHTS Universal Pro DNA Library Prep Kit for MGI

NDM608-01 24 rxn
EUR 907.2

VAHTS Universal Pro DNA Library Prep Kit for MGI

NDM608-02 96 rxn
EUR 2740.8

VAHTS Universal Plus DNA Library Prep Kit for MGI

NDM617-01 24 rxn
EUR 988.8

VAHTS Universal Plus DNA Library Prep Kit for MGI

NDM617-02 96 rxn
EUR 2889.6

1 Kb Ladder DNA Marker

FYD001-500UL 20 µg/ 500 µl Ask for price

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50 bp Ladder DNA Marker

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Universal platform

INC1112 EACH
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Universal Attachment

MIX1320 EACH
EUR 272.46

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)

ND607-03 24 rxn
EUR 514.8

VAHTS Universal DNA Library Prep Kit for Illumina V3 (PCR-Free)

ND607-04 96 rxn
EUR 1624.8

500-10000bp DNA Marker, Original Form

M101O-1 100loading, 100prep
EUR 110.11

500-10000bp DNA Marker, Original Form

M101O-2 5x100loading, 500prep
EUR 263.58

100-1500bp DNA Marker, Original Form

M102O-1 100loading, 100prep
EUR 108.02

100-1500bp DNA Marker, Original Form

M102O-2 5x100loading, 500prep
EUR 258.36

100-1500bp DNA Marker, Original Form

M107O-1 100loading, 100prep
EUR 110.11

100-1500bp DNA Marker, Original Form

M107O-2 5x100loading, 500prep
EUR 263.58

Image Green? 100 bp DNA Marker

M1155-500
EUR 352.8

Image Green? 1 kb DNA Marker

M1156-500
EUR 352.8

50-500bp Low Range DNA Marker

GM305 200loading, 200prep
EUR 137.78

2K Plus DNA Marker (8 linear double-stranded DNA bands)

20-abx098046
  • EUR 309.60
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  • 2.5 ml
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2K Plus DNA Marker (9 linear double-stranded DNA bands)

20-abx098047
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100bp Plus DNA Marker (12 linear double-stranded DNA bands)

20-abx098053
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  • EUR 226.80
  • 2.5 ml
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100bp Plus DNA Marker (14 linear double-stranded DNA bands)

20-abx098054
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  • EUR 243.60
  • 2.5 ml
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Universal Detachment Solution

AD002 100 ml
EUR 177.6

Universal Neutralization Buffer

NB001 100 ml
EUR 162

Universal Spring Platform

H1000-P-SP 1 PC
EUR 388.07

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QPCR1000-UNIV 4X1.25ml
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UNIVERSAL BEER AGAR

U21-100-10kg 10 kg
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U21-100-2kg 2kg
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UNIVERSAL BEER AGAR

U21-100-500g 500 g
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UNIVERSAL PREENRICHMENT BROTH

U21-101-10kg 10 kg
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UNIVERSAL PREENRICHMENT BROTH

U21-101-2Kg 2 Kg
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UNIVERSAL PREENRICHMENT BROTH

U21-101-500g 500 g
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Mains adaptor (universal)

ARG1248 EACH
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Grant Universal Tray

BAT4622 EACH
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BAT7177 EACH
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MIC6036 EACH
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MIX1080 EACH
EUR 171

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MIX1322 EACH
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MIX7184 EACH
EUR 738.72

Universal attachment IKA

SHA2210 EACH
EUR 744.42

NBS Universal Platform

SHA4050 EACH
EUR 1288.2

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SHA4070 EACH
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SHA4232 EACH
EUR 513

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SHA4256 EACH
EUR 628.14

NBS Universal Platform

SHA4272 EACH
EUR 996.36

NBS Universal Platform

SHA4290 EACH
EUR 1340.64

Universal AC adapter

PIP3214 EACH
EUR 62.93

Universal Multichannel Stand

F161417 EACH
EUR 28.5

Universal Oven UN30

OVE8314 EACH
EUR 1381.68

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OVE8316 EACH
EUR 1648.44

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OVE8318 EACH
EUR 1714.56

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OVE8320 EACH
EUR 2194.5

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OVE8322 EACH
EUR 2648.22

Universal Oven UN260

OVE8324 EACH
EUR 3505.5

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OVE8326 EACH
EUR 4639.8

Universal Oven UN750

OVE8328 EACH
EUR 6305.34

Universal Oven UF30

OVE8332 EACH
EUR 1614.24

Universal Oven UF55

OVE8334 EACH
EUR 1948.26

Universal Oven UF75

OVE8336 EACH
EUR 2056.56

Universal Oven UF110

OVE8338 EACH
EUR 2401.98

Universal Oven UF160

OVE8340 EACH
EUR 3161.22

Universal Oven UF260

OVE8342 EACH
EUR 4031.04

Universal Oven UF450

OVE8344 EACH
EUR 5278.2

Universal Oven UF750

OVE8346 EACH
EUR 7125

Universal Oven UF1060

OVE8348 EACH
EUR 12519.48

Universal Oven UN30plus

OVE8350 EACH
EUR 2020.08

Universal Oven UN55plus

OVE8352 EACH
EUR 2204.76

Universal Oven UN75plus

OVE8354 EACH
EUR 2453.28

Universal Oven UN110plus

OVE8356 EACH
EUR 2812.38

Universal Oven UN260plus

OVE8358 EACH
EUR 4189.5

Universal Oven UN450plus

OVE8360 EACH
EUR 5400.18

Universal Oven UN750plus

OVE8362 EACH
EUR 7083.96

Universal Oven UF30plus

OVE8366 EACH
EUR 2131.8

Universal Oven UF55plus

OVE8368 EACH
EUR 2530.8

Universal Oven UF75plus

OVE8370 EACH
EUR 2789.58

Universal Oven UF110plus

OVE8372 EACH
EUR 3218.22

Universal Oven UF160plus

OVE8374 EACH
EUR 4009.38

Universal Oven UF260plus

OVE8376 EACH
EUR 4753.8

Universal Oven UF450plus

OVE8378 EACH
EUR 6065.94

Universal Oven UF750plus

OVE8380 EACH
EUR 8708.46

Universal Oven UF1060plus

OVE8382 EACH
EUR 13331.16

18mmMag Universal ScrewCap

CHR3460 PK1000
EUR 950.76

Universal Indicator Solution

UN10025 2.5L
EUR 165.3

Universal Indicator Solution

UN1005 50ML
EUR 17.1

Universal Indicator Solution

UN101 100ML
EUR 18.24

Universal Indicator Solution

UN1025 25L
EUR 650.94

Universal Indicator Solution

UN105 500ML
EUR 90.06

Universal Indicator Solution

UNB1010 10L
EUR 627

IKA Universal Attachment

SHA2016 EACH
EUR 947.34

Universal V1-Puck

M-CP-111-021 1 PUCK
EUR 350
Description: Universal V1-Puck

Tumour Marker MUC1 (S1.1) , DNA Aptamer, Biotinylated

AD-152-B Custom Ask for price

Tumour Marker MUC1 (S1.1) , DNA Aptamer, unlabeled

AD-152-U Custom Ask for price

500-10000bp DNA Marker, Ready-to-use

M101R-1 100loading, 100prep
EUR 110.11

500-10000bp DNA Marker, Ready-to-use

M101R-2 5x100loading, 500prep
EUR 263.58

100-1500bp DNA Marker, Ready-to-use

M102R-1 100loading, 100prep
EUR 108.02

100-1500bp DNA Marker, Ready-to-use

M102R-2 5x100loading, 500prep
EUR 258.36

Lambda DNA/HindIII Marker, Ready-to-use

M104R-1 100loading, 100prep
EUR 78.79

Lambda DNA/HindIII Marker, Ready-to-use

M104R-2 5x100loading, 500prep
EUR 149.78

100-1500bp DNA Marker, Ready-to-use

M107R-1 100loading, 100prep
EUR 110.11

100-1500bp DNA Marker, Ready-to-use

M107R-2 5x100loading, 500prep
EUR 263.58

50-1500bp DNA Marker, Ready-to-use

M109-A 100loading, 100prep
EUR 110.11

50-1500bp DNA Marker, Ready-to-use

M109-B 5x100loading, 500prep
EUR 263.58

100-1200bp DNA Marker, Ready-to-use

GM333 50loading, 50prep
EUR 80.88

100-1200bp DNA Marker, Ready-to-use

GM334 5x50loading, 250prep
EUR 153.96

100-2000bp DNA Marker, Ready-to-use

GM335 50loading, 50prep
EUR 80.88

100-2000bp DNA Marker, Ready-to-use

GM336 5x50loading, 250prep
EUR 153.96

200-1500bp DNA Marker, Ready-to-use

GM337 50loading, 50prep
EUR 80.88

200-1500bp DNA Marker, Ready-to-use

GM338 5x50loading, 250prep
EUR 153.96

200-2000bp DNA Marker, Ready-to-use

GM339 50loading, 50prep
EUR 80.88

200-2000bp DNA Marker, Ready-to-use

GM340 5x50loading, 250prep
EUR 153.96

300-2500bp DNA Marker, Ready-to-use

GM341 50loading, 50prep
EUR 80.88

300-2500bp DNA Marker, Ready-to-use

GM342 5x50loading, 250prep
EUR 153.96

100-1000bp DNA Marker, Ready-to-use

GM343 50loading, 50prep
EUR 96.54

100-1000bp DNA Marker, Ready-to-use

GM345 50loading, 50prep
EUR 101.76

100-1500bp DNA Marker, Ready-to-use

SGM01 50loading, 50prep
EUR 80.88

100-1500bp DNA Marker, Ready-to-use

SGM02 5x50loading, 250prep
EUR 153.96

PCR plate-foil universal

A28216 1x100 pcs
EUR 112.8

PCR plate-foil universal

A28216L 5x100 pcs
EUR 372

Universal Casein Diluent/Blocker

85R-108 250 ml
EUR 276
Description: Ready-to-use biotin free reagent applicable for blocking ELISA plates

Universal Protein Precipitating Reagent

BSP012 100preps
EUR 97.58

5min Universal Ligation Mix

C311-01 50 rxn
EUR 256.8

5min Universal Ligation Mix

C311-02 100 rxn
EUR 339.6

Universal Full Growth Medium

TM001 500 ml
EUR 403.2

Universal Ohaus Plug GB

46001779 EACH
EUR 13.91

Universal tray for LSB12

BAT1062 EACH
EUR 263.34

Universal tray for LSB18

BAT1064 EACH
EUR 275.88

2 Universal Clip Plates

MIX5190 EACH
EUR 90.06

Azo Universal spray 500ml

CLE1996 EACH
EUR 5.18

Universal Active Charcoal Filter

FUM1146 EACH
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Universal Attachment for SHA20702

SHA2074 EACH
EUR 2370.06

IKA AS4000.1 Universal Attachment

SHA2612 EACH
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Universal Platform Innova S44i

SHA3072 EACH
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Grant Universal platform 270x195mm

SHA7932 EACH
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Universal Pipette Holder Assorted

PIP0164 EACH
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18mm Mag Universal ScrewCap

CHR3466 PK1000
EUR 898.32

18mm Mag Universal ScrewCap

CHR3468 PK1000
EUR 748.98

AED Universal Wall Bracket

SAF1128 EACH
EUR 84.93

100bp DNA Marker Plus-50, Ready-to-use

D1103-050 500 ul
EUR 181.2

100bp DNA Marker Plus-50, Ready-to-use

D1103-100 2x500 ul
EUR 230.4

100bp PCR Ranger DNA Marker, 100bp-To-3Kb

D1108-100 1ml
EUR 264

100bp PCR Ranger DNA Marker, 100bp-To-3Kb

D1108-200 2x1ml
EUR 370.8

1Kb PCR Ranger DNA Marker, 750bp-To-20Kb

D1109-100 1ml
EUR 225.6

Tumour Marker MUC1 (S1.1) , DNA Aptamer, FITC labeled

AD-152-F Custom Ask for price

200-2000bp DNA Marker Plus, Ready-to-use

GM401 50loading, 50prep
EUR 72.53

100-5000bp DNA Marker Plus, Ready-to-use

SGM03 50loading, 50prep
EUR 80.88

1Kb PCR Ranger DNA Marker, 750bp-To-20Kb

D1109-400 4x1ml
EUR 465.6

Universal IHC Diluent/Blocker/Stabilizer

85R-1021 1 liter
EUR 830.4
Description: Universal Diluent/Blocker/Stabilizer for use in IHC

HotTaq Probe qPCR Universal Mix

BT11004 250rxn
EUR 147.6
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Universal Mix

BT11104 250rxn
EUR 165.6
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

UNIVERSAL LINEAR 4 PLACE STAND

AP-USD4 1/pk
EUR 84
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

VAHTS Universal Plus Fragmentation Module

N209-01 24 rxn
EUR 451.2

VAHTS Universal Plus Fragmentation Module

N209-02 96 rxn
EUR 1226.4

Universal 3' miRNA Reverse Primer

MPH00000 150 ul / 10 uM
EUR 84

10 x Universal Blocking Buffer

GR103005 200 mL
EUR 154.8

100UL UNIVERSAL FIT FILTER TIPS

TF-100 1000/pk
EUR 501.6
Description: Filter Tip; Filter Tips Pipette - Axygen

1000UL UNIVERSAL FIT FILTER TIPS

TF-1000 1000/pk
EUR 278.4
Description: Filter Tip; Filter Tips Pipette - Axygen

200UL UNIVERSAL FIT FILTER TIPS

TF-200 1000/pk
EUR 501.6
Description: Filter Tip; Filter Tips Pipette - Axygen

300UL UNIVERSAL FIT FILTER TIPS

TF-350 1000/pk
EUR 501.6
Description: Filter Tip; Filter Tips Pipette - Axygen

Subsequently, the mixture of thermal therapy, molecular focused therapy, and gene therapy synergistically targets GLC-82 cells, and the usage of C225-IFNG-IMANS as a gene or drug provider provides a novel and promising strategy for the therapy of lung most cancers.

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