Doped graphite-like coating (GLC) has aroused nice curiosity as one of the crucial promising protecting supplies in marine functions. Nonetheless, there’s a lack of systematic analysis on the tribocorrosion and antifouling efficiency of doped GLC coatings in harsh marine environments. Herein, a multifunctional (Cr, Cu)-GLC coating with mixed antifouling and tribocorrosion properties was ready through a magnetron sputtering methodology. The experimental outcomes point out that the resultant coatings modified from a dense construction to a free columnar construction with the increment of Cr and Cu doping quantity.
On the similar time, the hardness of the coating steadily decreases, however the contact angle between coating and seawater steadily will increase. The algae adhesion check reveal that the algae density on the floor of the (Cr, Cu)-GLC coating decreases from about 565 to 70/mm2 as the quantity of doping elevated. Nonetheless, quite the opposite, the friction coefficient of the coating below OCP situation will increase from 0.06 to about 0.35. General, the gentle doped (Cr, Cu)-GLC coating reveals the very best complete properties, combining antifouling and tribocorrosion properties. The corresponded mechanisms are mentioned when it comes to the coating microstructure, antifouling, and tribocorrosion conduct.
The corrosion and tribology are all carefully associated to the interface/floor of supplies, that are extraordinarily vital for the mechanical elements utilized in harsh marine environments. On this work, we fabricated Cr/graphite-like carbon (GLC) multilayered movies with totally different modulation durations on the 316L stainless steels by direct present magnetron sputtering. Tribocorrosion assessments in synthetic seawater present that the tribocorrosion resistance of the Cr/GLC movies is improved because the modulation interval decreases from 1000 to 333 nm after which drastically drops with additional lowering to 250 nm. By taking a top-layer thickening technique for the Cr/GLC movie with 250 nm modulation interval, the tribocorrosion efficiency is considerably enhanced. The corresponded mechanisms are mentioned when it comes to the movie construction and electrochemical corrosion conduct.
Immunomodulatory glc/man-directed Dolichos lablab lectin (DLL) evokes anti-tumour response in vivo by counteracting angiogenic gene expressions.
Neovascularization and jeopardized immunity has been critically emphasised for the institution of malignant development. Lectins are the varied class of carbohydrate interacting proteins, having nice potential as immunopotentiating and anti-cancer brokers. The current investigation sought to exhibit the anti-proliferative exercise of Dolichos lablab lectin (DLL) encompassing immunomodulatory attributes. DLL particular to glucose and mannose carbohydrate moieties has been purified to homogeneity from the widespread dietary legume D. lablab. Outcomes elucidated that DLL agglutinated blood cells non-specifically and displayed placing mitogenicity to human and murine lymphocytes in vitro with interleukin (IL)-2 manufacturing.
The DLL-conditioned medium exerted cytotoxicity in direction of malignant cells and neoangiogenesis in vitro. Equally, in-vivo anti-tumour investigation of DLL elucidated the regressed proliferation of ascitic and strong tumour cells, which was paralleled with blockade of tumour neovasculature. DLL-treated mice confirmed an up-regulated immunoregulatory cytokine IL-2 in distinction to severely declined ranges in management mice. Mechanistic validation revealed that DLL has abrogated the microvessel formation by weakening the proangiogenic alerts, particularly nuclear issue kappa B (NF-κB), hypoxia inducible issue 1α (HIF-1 α), matrix metalloproteinase (MMP)-2 and 9 and vascular endothelial development issue (VEGF) in malignant cells resulting in tumour regression.
In abstract, it’s evident that the dietary lectin DLL probably dampens the malignant institution by mitigating neoangiogenesis and immune shutdown. For the primary time, to our data, this research illustrates the important position of DLL as an immunostimulatory and anti-angiogenic molecule in most cancers therapeutics.
Alteration of cell wall polysaccharides by way of transgenic expression of UDP-Glc 4-epimerase-encoding genes in potato tubers.
Uridine diphosphate (UDP)-glucose 4-epimerase (UGE) catalyzes the conversion of UDP-glucose to UDP-galactose. Cell wall supplies from the cv. Kardal (wild-type, background) and two UGE transgenic traces (UGE 45-1 and UGE 51-16) have been remoted and fractionated. The galactose (Gal) content material (mg/100g tuber) from UGE 45-1 transgenic line was 38% greater than that of wild-type, and resulted in longer pectin facet chains. The Gal content material current in UGE 51-16 was 17% decrease than that of wild-type, though most pectin populations maintained the identical degree of Gal.
Each UGE transgenic traces confirmed unexpectedly a lower in acetylation and a rise in methyl-esterification of pectin. Each UGE transgenic traces confirmed related proportions of homogalacturonan and rhamnogalacturonan I inside pectin spine because the wild-type, apart from the calcium-bound pectin fraction exhibiting comparatively much less rhamnogalacturonan I. Subsequent to pectin modification, xyloglucan populations from each transgenic traces have been altered leading to totally different XSGG and XXGG proportion compared to wild-type.
Mode of Interplay of the Sign-Transducing Protein EIIA(Glc) with the Maltose ABC Transporter within the Technique of Inducer Exclusion.
Enzyme IIA(Glc) (EIIA(Glc)) of the phosphoenolpyruvate phosphotransferase system for the uptake of glucose in Escherichia coli and Salmonella inhibits the maltose ATP-binding cassette transporter (MalE-FGK2) by interplay with the nucleotide-binding and -hydrolyzing subunit MalK, a course of termed inducer exclusion. We’ve got investigated binding of EIIA(Glc) to the MalK dimer by cysteine cross-linking in proteoliposomes. The outcomes show that the binding website I of EIIA(Glc) is contacting the N-terminal subdomain of MalK whereas the binding website II is comparatively near the C-terminal (regulatory) subdomain, in settlement with a crystal construction [ Chen , S. , Oldham , M. L. , Davidson , A. L. , and Chen , J. ( 2013 ) Nature 499 , 364 – 368 ].
The egg drop syndrome (76) PCR kit |
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PCR-V044-48D | Bioingentech | 50T | EUR 543.6 |
The egg drop syndrome (76) PCR kit |
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PCR-V044-96D | Bioingentech | 100T | EUR 686.4 |
Egg Drop Syndrome One-Step PCR kit |
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Oneq-V026-100D | Bioingentech | 100T | EUR 1039.2 |
Egg Drop Syndrome One-Step PCR kit |
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Oneq-V026-150D | Bioingentech | 150T | EUR 1177.2 |
Egg Drop Syndrome One-Step PCR kit |
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Oneq-V026-50D | Bioingentech | 50T | EUR 861.6 |
The egg drop syndrome (76) RT PCR kit |
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RTq-V044-100D | Bioingentech | 100T | EUR 860.4 |
The egg drop syndrome (76) RT PCR kit |
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RTq-V044-150D | Bioingentech | 150T | EUR 969.6 |
The egg drop syndrome (76) RT PCR kit |
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RTq-V044-50D | Bioingentech | 50T | EUR 717.6 |
Probe PCR Mix |
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abx461071-1096tests | Abbexa | 10 × 96 tests | Ask for price |
Probe PCR Mix |
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abx461071-596tests | Abbexa | 5 × 96 tests | Ask for price |
Probe PCR Mix |
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abx461071-96tests | Abbexa | 96 tests | EUR 1437.5 |
Ready? PCR Mix |
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M1127-1000 | Biovision | each | EUR 614.4 |
Ready? PCR Mix |
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M1127-200 | Biovision | each | EUR 229.2 |
Rigor? PCR Mix |
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M1132-200 | Biovision | each | EUR 385.2 |
Breeze? PCR Mix |
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M1134-200 | Biovision | each | EUR 320.4 |
Distant? PCR Mix |
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M1136-200 | Biovision | each | EUR 352.8 |
Advance? PCR Mix |
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M1139-200 | Biovision | each | EUR 451.2 |
The egg drop syndrome (76) One-Step PCR kit |
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Oneq-V044-100D | Bioingentech | 100T | EUR 1039.2 |
The egg drop syndrome (76) One-Step PCR kit |
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Oneq-V044-150D | Bioingentech | 150T | EUR 1177.2 |
The egg drop syndrome (76) One-Step PCR kit |
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Oneq-V044-50D | Bioingentech | 50T | EUR 861.6 |
PCR Super Mix |
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abx299726-100g | Abbexa | 100 µg | Ask for price |
PCR Super Mix |
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abx299726-20g | Abbexa | 20 µg | EUR 362.5 |
PCR Super Mix |
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abx299726-50g | Abbexa | 50 µg | Ask for price |
Drop-out Mix Complete w/o Yeast Nitrogen Base (Powder) |
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MBS652672-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Complete w/o Yeast Nitrogen Base (Powder) |
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MBS652672-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Complete w/o Yeast Nitrogen Base (Powder) |
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MBS652672-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Complete w/o Yeast Nitrogen Base (Powder) |
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MBS652672-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Complete w/o Yeast Nitrogen Base (Powder) |
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MBS652672-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Ready PCR Mix 2X |
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11170052-1 | Bio-WORLD | 1 Unit (s) | EUR 56.92 |
Ready PCR Mix 2X |
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11170052-2 | Bio-WORLD | 2 Unit (s) | EUR 103.49 |
Fire Start? PCR Mix |
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M1141-200 | Biovision | each | EUR 451.2 |
Ready? PCR Mix-Dye |
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M1128-1000 | Biovision | each | EUR 614.4 |
Ready? PCR Mix-Dye |
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M1128-200 | Biovision | each | EUR 229.2 |
Image Ready? PCR Mix |
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M1129-200 | Biovision | each | EUR 352.8 |
Rigor? PCR Mix-Dye |
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M1133-200 | Biovision | each | EUR 385.2 |
Whole Blood PCR Mix |
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M1143-200 | Biovision | each | EUR 385.2 |
PowerPol 2X PCR Mix |
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RK20718 | Abclonal | 1mL | EUR 23.44 |
Robust Ready? PCR Mix |
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M1130-1000 | Biovision | each | EUR 738 |
Robust Ready? PCR Mix |
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M1130-200 | Biovision | each | EUR 255.6 |
Breeze? PCR Mix-Dye |
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M1135-200 | Biovision | each | EUR 320.4 |
Drop-out Mix Minus Lysine w/o Yeast Nitrogen Base (Powder) |
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MBS652781-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Minus Lysine w/o Yeast Nitrogen Base (Powder) |
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MBS652781-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Minus Lysine w/o Yeast Nitrogen Base (Powder) |
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MBS652781-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Minus Lysine w/o Yeast Nitrogen Base (Powder) |
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MBS652781-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Minus Lysine w/o Yeast Nitrogen Base (Powder) |
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MBS652781-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Taq Mix (2x) (PCR Master Mix (2x)) |
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50900-1 | Sisco Laboratories | 5 x1 ml | EUR 64.14 |
Description: Part E |
Taq Mix (2x) (PCR Master Mix (2x)) |
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50900-2 | Sisco Laboratories | 5 x2.5 ml | EUR 128.29 |
Description: Part E |
Distant? PCR Mix-Dye |
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M1137-200 | Biovision | each | EUR 352.8 |
Image Distant? PCR Mix |
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M1138-200 | Biovision | each | EUR 385.2 |
Advance? PCR Mix-Dye |
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M1140-200 | Biovision | each | EUR 451.2 |
2x PCR Red Mix |
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TBS4004 | Tribioscience | 2.0 ml | EUR 88 |
2x PCR Blue Mix |
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TBS4005 | Tribioscience | 2.0 ml | EUR 88 |
PCR-EZ D-PCR MASTER MIX |
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BS294 | Bio Basic | 100RXN, 100prep | EUR 112.2 |
2X PCR Master Mix |
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28007 | Norgen Biotek Corp | 100 Reactions | EUR 47.4 |
2X PCR Master Mix |
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28009 | Norgen Biotek Corp | 500 Reactions | EUR 209.4 |
PCR Master Mix 2X |
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11140011-1 | Bio-WORLD | 100 Reaction (s) | EUR 210.3 |
PCR Master Mix 2X |
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11140011-2 | Bio-WORLD | 200 Reaction (s) | EUR 396.6 |
PCR Master Mix 2X |
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11140011-3 | Bio-WORLD | 500 Reaction (s) | EUR 849.23 |
Drop-out Mix Minus Phenylalanine w/o Yeast Nitrogen Base (Powder) |
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MBS653410-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Minus Phenylalanine w/o Yeast Nitrogen Base (Powder) |
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MBS653410-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Minus Phenylalanine w/o Yeast Nitrogen Base (Powder) |
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MBS653410-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Minus Phenylalanine w/o Yeast Nitrogen Base (Powder) |
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MBS653410-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Minus Phenylalanine w/o Yeast Nitrogen Base (Powder) |
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MBS653410-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
HRM PCR Master Mix |
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E0440-01 | EURx | 100reactions | EUR 55.59 |
Description: For detection of gene mutations and SNPs by HRM analysis |
HRM PCR Master Mix |
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E0440-02 | EURx | 500reactions | EUR 251.79 |
Description: For detection of gene mutations and SNPs by HRM analysis |
MGI PCR Primer Mix |
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RK20272 | Abclonal | 40μL | EUR 6 |
PCR Master mix (SYBR) |
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abx460031-1096tests | Abbexa | 10 × 96 tests | Ask for price |
PCR Master mix (SYBR) |
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abx460031-596tests | Abbexa | 5 × 96 tests | Ask for price |
PCR Master mix (SYBR) |
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abx460031-96tests | Abbexa | 96 tests | EUR 337.5 |
amfiSure PCR Master Mix |
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P0311-010 | GenDepot | 2x50 rxns | EUR 66 |
amfiSure PCR Master Mix |
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P0311-025 | GenDepot | 5X50 rxns | EUR 99 |
amfiSure PCR Master Mix |
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P0311-050 | GenDepot | 10X50 rxns | EUR 149 |
amfiSure PCR Master Mix |
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P0311-100 | GenDepot | 10x1ml | EUR 220 |
amfiSure PCR Master Mix |
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P0311-125 | GenDepot | 15X50 rxns | EUR 198 |
amfiSure PCR Master Mix |
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P0311-200 | GenDepot | 20x1ml | EUR 365 |
amfiSure PCR Master Mix |
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P0311-250 | GenDepot | 50x50 rxns | EUR 595 |
amfiSure PCR Master Mix |
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P0311-500 | GenDepot | 100x50 rxns | EUR 1010 |
PCR-Mix |
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L5051100 | Biochain | 2.5 ml | EUR 52.5 |
amfiXpand PCR Master Mix |
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P0331-010 | GenDepot | 2X50 rxns | EUR 65 |
amfiXpand PCR Master Mix |
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P0331-025 | GenDepot | 5X50 rxns | EUR 118 |
amfiXpand PCR Master Mix |
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P0331-050 | GenDepot | 10X50 rxns | EUR 149 |
amfiXpand PCR Master Mix |
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P0331-250 | GenDepot | 50x50 rxns | EUR 430 |
Taq 2X PCR Mix V2 |
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RK20607 | Abclonal | 5mL | EUR 3.82 |
Roseate PCR Master Mix (2x) (Taq Mix (2x)) |
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46503 | Sisco Laboratories | 1 ml | EUR 23.09 |
Description: Part E |
Roseate PCR Master Mix (2x) (Taq Mix (2x)) |
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46503-1 | Sisco Laboratories | 5 x1 ml | EUR 70.13 |
Description: Part E |
Roseate PCR Master Mix (2x) (Taq Mix (2x)) |
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46503-2 | Sisco Laboratories | 5 x2.5 ml | EUR 145.39 |
Description: Part E |
Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Powder) |
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MBS652948-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Powder) |
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MBS652948-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Powder) |
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MBS652948-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Powder) |
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MBS652948-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Powder) |
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MBS652948-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
MyTaq Blood PCR Mix, 2x |
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BIO-25053/S | Bioline | Sample | Ask for price |
MyTaq Blood PCR Mix, 2x |
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BIO-25054 | Bioline | 250 Reactions | Ask for price |
Taq 2x PCR Mix 100 rxn |
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BA01501 | Bioatlas | 100rxn | EUR 112.8 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base (Powder) |
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MBS653231-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base (Powder) |
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MBS653231-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base (Powder) |
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MBS653231-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base (Powder) |
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MBS653231-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Leucine w/o Yeast Nitrogen Base (Powder) |
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MBS653231-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Drop-out Mix Synthetic Minus Adenine w/o Yeast Nitrogen Base (Powder) |
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MBS653424-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Adenine w/o Yeast Nitrogen Base (Powder) |
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MBS653424-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Adenine w/o Yeast Nitrogen Base (Powder) |
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MBS653424-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Adenine w/o Yeast Nitrogen Base (Powder) |
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MBS653424-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Adenine w/o Yeast Nitrogen Base (Powder) |
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MBS653424-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Fire Start? PCR Mix-Dye |
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M1142-200 | Biovision | each | EUR 451.2 |
HiScript-TS 2 × PCR Mix |
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RA103-01 | Vazyme | 40 rxns (25 μl/rxn) | EUR 87 |
HiScript-TS 2 × PCR Mix |
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RA103-02 | Vazyme | 200 rxns (25 μl/rxn) | EUR 344 |
Drop-out Mix Synthetic Minus Arginine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653068-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Arginine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653068-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Arginine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653068-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Arginine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653068-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Arginine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653068-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
HotTaq 2x PCR Mix 100 rxn |
|||
BA01503 | Bioatlas | 100rxn | EUR 144 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
RedTaq 2x PCR Mix 100 rxn |
|||
BA01507 | Bioatlas | 100rxn | EUR 175.2 |
Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
Drop-out Mix Synthetic Minus Histidine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652765-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Histidine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652765-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Histidine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652765-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Histidine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652765-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Histidine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652765-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Drop-out Mix Synthetic Minus Threonine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653230-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Threonine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653230-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Threonine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653230-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Threonine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653230-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Threonine w/o Yeast Nitrogen Base (Powder) |
|||
MBS653230-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Robust Ready? PCR Mix-Dye |
|||
M1131-1000 | Biovision | each | EUR 738 |
Robust Ready? PCR Mix-Dye |
|||
M1131-200 | Biovision | each | EUR 255.6 |
Drop-out Mix Synthetic Minus Methionine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652762-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Methionine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652762-1kg | MyBiosource | 1kg | EUR 1180 |
Drop-out Mix Synthetic Minus Methionine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652762-25kg | MyBiosource | 2.5kg | EUR 2340 |
Drop-out Mix Synthetic Minus Methionine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652762-500g | MyBiosource | 500g | EUR 825 |
Drop-out Mix Synthetic Minus Methionine w/o Yeast Nitrogen Base (Powder) |
|||
MBS652762-5x25kg | MyBiosource | 5x2.5kg | EUR 10260 |
Drop-out Mix Synthetic Minus Tryptophan w/o Yeast Nitrogen Base (Powder) |
|||
MBS653447-100g | MyBiosource | 100g | EUR 260 |
Drop-out Mix Synthetic Minus Tryptophan w/o Yeast Nitrogen Base (Powder) |
|||
MBS653447-1kg | MyBiosource | 1kg | EUR 1180 |
Furthermore, EIIA(Glc) was discovered to bind to the MalK dimer no matter its conformational state. Deletion of the amphipathic N-terminal peptide of EIIA(Glc), which is required for inhibition, diminished formation of cross-linked merchandise. Utilizing a spin-labeled transporter variant and EPR spectroscopy, we exhibit that EIIA(Glc) arrests the transport cycle by inhibiting the ATP-dependent closure of the MalK dimer.